HALO
Hyper-fast, super rugged (U)HPLC columns
The fused-core* particle technology was specially
developed to produce hyper-fast chromatographic separation while avoiding
the uncertainties associated with UHPLC. The father of the technique is dr.
Jack Kirkland, widely regarded as one of the "founders" of HPLC.
* or core-shell, or
superficially-porous, or semi-porous, or pellicular
BACK PRESSURE - significantly less compared to UHPLC
columns
CONVENTIONAL HPLC EQUIPMENT can be used with near-UHPLC performance
2um INLET FRIT eliminates frit plugging problem
FAST SEPARATION at high flow rates maintaining the resolving power
HALO columns for small molecules
| Phase | Particle (um) | Pore (A) | Carbon load (%) | pH limit |
C8 |
2 2.7 5 |
90 |
4.8 5.4 3.7 |
2 - 9 |
C18 |
2 2.7 5 |
90 |
7.2 7.7 5.4 |
2 - 9 |
C18 (AQ) |
2 2.7 5 |
90 |
6.5 / 6.7 |
2 - 9 |
| C30 | 2.7 |
160 | 4.5 |
2 - 9 |
| Biphenyl | 2.7 |
90 | 7.0 |
2 - 9 |
Phenyl-Hexyl |
2 2.7 5 |
90 |
6.3 7.1 5.2 |
2 - 9 |
RP-Amide |
2 2.7 5 |
90 |
7.3 8.2 5.1 |
2 - 9 |
PFP |
2 2.7 5 |
90 |
5.3 5.5 3.9 |
2 - 8 |
| PAH | 2.7 |
90 | 9.9 |
2 - 9 |
| PFAS | 2.7 |
90 | 9.9 |
2 - 9 |
ES-CN |
2 2.7 5 |
90 |
3.4 3.5 2.5 |
1 - 8 |
HILIC |
2 2.7 5 |
90 |
unbonded |
1 - 8 |
Penta-HILIC |
2 2.7 5 |
90 |
2.8 3.2 2.1 |
2 - 9 |
Chemistries
 Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes. |
 Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes. |
 Resistant to dewetting and compatible with 100% aqueous mobile phases. Retains polar molecules more than classic C18 phases. |
 High shape selectivity for hydrophobic, long-chain, structurally related isomers. 100% aqueous compatibility. |
 Separation of polar compounds with a combination of hydrophobic, aromatic, and polar selectivities. |
 Separations occur due to hydrophobic and pi-pi interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens). Compatible with highly aqueous mobile phases. |
 Retention and selectivity due to hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases. |
 Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, pi-pi, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode (with more than 80% acetonitrile in mobile phase). Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases. |
 This trifunctional, un-endcapped C18 bonded phase has been specifically designed to provide fast, efficient, selective separations of PAH compounds. |
 Moderately polar phase for alternate selectivity. Steric-protected silane for low pH stability. Fully end-capped for neutral pH applications. |
 Can be used in both HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes. |
 Provides superior retention to silica HILIC and amide HILIC phases for acids and zwitterionic analytes. |
| Phase | Particle (um) | Pore (A) | Carbon load (%) | pH limit |
| C4 | 3.4 | 400 | 0.4 | 2 - 9 |
| C4 | 2.7 | 1000 | 0.6 | 2 - 9 |
ES-C18 |
2 2.7 5 |
160 |
4.0 4.6 4.0 |
1 - 8 |
| ES-C18 | 3.4 | 400 | 1.0 | 1 - 8 |
| ES-C18 | 2.7 | 1000 | 1.4 | 1 - 8 |
| Diphenyl | 2.7 | 1000 | 0.9 | 2 - 9 |
| Phenyl-Hexyl | 2.7 | 160 | 4.7 | 2 - 9 |
| ES-CN | 2.7 5 |
160 | 2.2 1.5 |
1 - 8 |
| Glycan | 2.7 | 90 | 3.2 | 2 - 9 |
Chemistries
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HALO Guard system
AMT use the Optimize Technologies
EXP Direct Connect Holder
KEY FEATURES
- Hand-tight cartridge replacement
- Hardware rated to 20,000+ psi (1,400+ bar)
- 1.4uL Swept Volume (excluding packed bed)
- Auto-adjusting ZDV column connection
- Hardened stainless steel end cap eliminates galling
- Features Free-Turn architecture
- For use with EXP Trap and Guard Cartridges
- Fittings included
News
HALO PAH columns: this
trifunctional C18 bonded phase provides fast, efficient, selective separation of
PAH compounds.
HALO 90A PAH Columns
HALO PFAS columns: developed for
the analysis of short-chain and long-chain PFAS (per- and polyfluoroalkyl
substances)
HALO 90A PFAS Columns
Catalogs, Guides
HALO General Catalog
HALO Enviro-class Catalog
HALO Method Conversion
Guidebook
HALO Guidebook on Reverse Phase
Chemistries
HALO Small Molecule
Method Development Guide
HALO Bioclass Columns
HALO Bioanalysis Guidebook
HALO Biotherapeutic
Method Development Guide
Brochures, Care-and-Use Sheets
Maintaining LC Column
Performance
HALO 2um Columns
HALO 5um Columns
HALO Guard Cartridges
Posters, Presentations
New Fused-Core Particles for Very
Fast HPLC Separations (Article)
New Fused-Core Particles for Very
Fast HPLC Separations (Poster)
Take Your UHPLC and HPLC Methods to
the Next Level of Speed and Resolution
Nano-Capillary HPLC with Fused-core Particles
for Proteomics Applications
Analysis of Lipids by HPLC (HALO C8)
Converting Conventional RP-HPLC
Separations to Ultra-Fast Separations
Bridge the Gap between UHPLC and HPLC
Modifying Agilent 1100 HPLC Systems to
Achieve "UHPLC-like" Performance
HALO HILIC 2.7 and 5um Column Comparison
HALO Penta-HILIC 2.7 and 5um Column Comparison
HALO 1000As: Selectivity
Manipulation for LC/MS Analysis of Protein Variants
HALO 90A AQ-C18: LC/MS
Method for the Analysis of Guanine Deaminase
Applications
Articles
LC-GC Europe
Magazine:
Joseph Jack Kirkland - the HPLC Particle Pioneer
Understanding and Overcoming
Separation Challenges in the Biological Drug Development Process
Environmental Analyses of Harmful
Chemicals
Importance of Particle Pore Size in
Determining Retention and Selectivity in Reversed Phase Liquid Chromatography
Optimizing Particle Pores
for HPLC Separation of Verapamil and other Mid Size Molecules
Selection of HPLC and LC/MS
Conditions for the Separation of Polar Compounds and Protein Modifications
Technical Reports
LC-MS/MS methods for the analysis of
short-chain and long-chain PFAS
HALO resolution, sensitivity and
stability for pharmaceutical method screening
Fat- and water-soluble vitamin analysis
in supplements
 Extra column volume |
 HALO capillary column instructions |
