HALO

 

News

Fortis SpeedCore pH+ C18
pH 1 - 12 range

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Promotion

HALO Selectivity Kits:
3 columns for the price of 1!

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Events

ISC-2018 (Cannes-Mandelieu, France)

23-27 September, 2018

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... the original fused-core particles

Hyper-fast, super rugged HPLC columns

The fused-core* particle technology was specially developed to produce hyper-fast chromatographic separation while avoiding the uncertainties associated with UHPLC. The father of the technique is dr. Jack Kirkland, widely regarded as one of the "founders" of HPLC.
     * or core-shell, or superficially-porous, or semi-porous, or pellicular

Fused-core particle

BACK PRESSURE - significantly less compared to UHPLC columns
CONVENTIONAL HPLC EQUIPMENT can be used with near-UHPLC performance
2um INLET FRIT eliminates frit plugging problem
FAST SEPARATION at high flow rates maintaining the resolving power

Fast separation (JPEG file, opens in new window)

 

HALO 2.7um Specifications

- Ultra-pure type "B" silica support
- 1.7um solid core with 0.5um porous silica layer fused to the surface
- 150 m2/g surface
- 90A or 1000A (C4) pore size

- Monomeric bonding chemistry
- pH range: 2 to 9
- Max. pressure: 600 bar

Chemistries

C4 ... The world’s first 2.7-micron superficially porous silica particles with 1000 Angstrom pores for the separation of very large molecules such as monoclonal antibodies, large proteins, synthetic polymers, etc.
C8 ... Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes.
C18 ... Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes.
Peptide ES-C18 ... Ideal for ultrafast and high resolution separation of peptides and polypeptides ( 15 kDa). Enhanced stability at low pH via sterically protected ligands up to 90 degrees C.
Phenyl-Hexyl ... Separations occur due to hydrophobic and pi-pi interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens). Compatible with highly aqueous mobile phases.
HILIC ... Can be used in both HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes.
Penta-HILIC ... Provides superior retention to silica HILIC and amide HILIC phases for acids and zwitterionic analytes
PFP ... Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, pi-pi, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode (with more than 80% acetonitrile in mobile phase). Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
RP-Amide ... Retention and selectivity due to hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases.
Peptide ES-CN ... Provides strong dipole-dipole interactions with analytes and weak hydrophobic interactions. Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid equilibration with low bleed for high throughput LC-MS applications. Extra stable bonding with sterically protected ligand provides improved stability and performance compared to conventional CN phases, especially at low pH (1-4).

 

HALO 3.4um Specifications

- Ultra-pure type "B" silica support
- 3.0um solid core with 0.2um porous silica layer fused to the surface
- 400A pore size

Chemistries

Protein C4 ... Dimethylbutylsilane, exhaustively endcapped and enhanced for high temperature stability.
Protein ES-C18 ... Reversed-phase wide-pore packing for separating mid-high molecular weight compounds such as proteins and polypeptides < 500 kDa

 

HALO 5um Specifications

- Ultra-pure type "B" silica support
- 3.8um solid core with 0.6um porous silica layer fused to the surface
- 90 m2/g surface (equivalent to 150-200m2/g totally porous particles)
- 90A pore size

- Monomeric bonding chemistry
- pH range: 2 to 9
- Max. pressure: 600 bar

Chemistries

C8 ... Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes.
C18
... Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes.
Peptide ES-C18 ... Ideal for ultrafast and high resolution separation of peptides and polypeptides ( 15 kDa). Enhanced stability at low pH via sterically protected ligands up to 90 degrees C.
Phenyl-Hexyl ... Separations occur due to hydrophobic and pi-pi interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens).
HILIC ... Can be used in both HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes.
Penta-HILIC ... Provides superior retention to silica HILIC and amide HILIC phases for acids and zwitterionic analytes
PFP ... Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, pi-pi, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode (with more than 80% acetonitrile in mobile phase). Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
RP-Amide ... Retention and selectivity due to hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases.
Peptide ES-CN ... Provides strong dipole-dipole interactions with analytes and weak hydrophobic interactions. Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid equilibration with low bleed for high throughput LC-MS applications. Extra stable bonding with sterically protected ligand provides improved stability and performance compared to conventional CN phases, especially at low pH (1-4).

HALO Bioclass Packings

Packing Description Bonded Phase Retention Mode Target Analytes
HALO Peptide ES-C18 Peptide ES-C18
(diisobutyloctadecylsilane)
Hydrophobic interactions A stable, reversed-phase packing used for separating peptides and polypeptides < 20 kDa
HALO Peptide ES-CN Peptide ES-CN
(diisopropylcyanopropylsilane)
Hydrophobic interactions An alternative stable, reversed-phase packing that can be used for separating peptides and polypeptides < 20 kDa
HALO Protein C4 Protein C4
(dimethylbutylsilane)
Hydrophobic interactions A wide-pore stable, reversed-phase packing used for separating high molecular weight compounds such as proteins and polypeptides < 500 kDa
HALO Protein ES-C18 Protein ES-C18
(diisobutyloctadecylsilane)
Hydrophobic interactions An alternative stable, reversed-phase wide-pore packing for separating mid-high molecular weight compounds such as proteins and polypeptides < 500 kDa
HALO Glycan Glycan proprietary
penta-hydroxy-ligand
Hydrophilic interaction
polyhydroxy bonded phase
w. adsorbed aqueous layer
Ideal for separation of highly polar oligosaccharides and particularly for protein-linked glycans and glycopeptides

Packing Description Endcapped Particle, um Pore, A Max P (bar) Max T (°C) pH Carbon, %
HALO Peptide ES-C18 No 2 / 2.7 / 5 160 600 90 1 - 8 4.6 / 4.0
HALO Peptide ES-CN Yes 2.7 / 5 160 600 90 1 - 8 2.2 / 1.5
HALO Protein C4 Yes 3.4 400 600 90 2 - 9 0.4
HALO Protein ES-C18 Yes 3.4 400 600 90 1 - 8 1.0
HALO Glycan No 2.7 90 600 65 2 - 9 3.2
HALO C4 1000 Yes 2.7 1000 600 40/90 2 - 9 0.6
HALO ES-C18 1000 Yes 2.7 1000 600 40/90 1 - 8 1.4
HALO Diphenyl 1000 Yes 2.7 1000 600 40/90 2 - 9 1.0

 

 

 

Downloads

News

HALO Toolbox: small molecule and protein selectivity kits
HALO Small Molecule Method Development Guide
HALO Biotherapeutic Method Development Guide

Catalogs

HALO Catalog
HALO Bioclass Columns

 Brochures

HALO HILIC Columns
HALO HILIC Column Care
HALO Penta-HILIC Columns
HALO PFP Columns
HALO PFP Column Care
HALO PhenylHexyl Columns
HALO RP-Amide Columns
HALO Guard Cartridges
Eternity PH HALO Peptide ES-C18
HALO Protein and Peptide Columns
HALO ES-CN flyer
HALO 5um Columns
HALO-5 C8 Column Care
HALO-5 ES-CN Column Care
HALO-5 PhenylHexyl Column Care
HALO-5 HILIC Column Care
HALO-5 Penta-HILIC Column Care
HALO-5 RP-Amide Column Care
HALO 1000A C4 Columns
HALO 1000A Diphenyl Columns
HALO 2um ES-C18 Peptide Columns
HALO 2um Columns
HALO 160A Phenyl-Hexyl Columns
HALO 90A Biphenyl Columns
HALO 90A AQ-C18 Columns

 Posters, Presentations

New Fused-Core Particles for Very Fast HPLC Separations (Article)
New Fused-Core Particles for Very Fast HPLC Separations (Poster)
Nano-Capillary HPLC with Fused-core Particles for Proteomics Applications
Analysis of Lipids by HPLC (HALO C8)
Converting Conventional RP-HPLC Separations to Ultra-Fast Separations

Bridge the Gap between UHPLC and HPLC
Modifying Agilent 1100 HPLC Systems to Achieve "UHPLC-like" Performance

HALO HILIC 2.7 and 5um Column Comparison
HALO Penta-HILIC 2.7 and 5um Column Comparison
HALO 1000As: Selectivity Manipulation for LC/MS Analysis of Protein Variants
HALO 90A AQ-C18: LC/MS Method for the Analysis of Guanine Deaminase

Applications

AN001 NSAIDS on HALO C18
AN002 NSAIDS on HALO C8
AN003 NSAIDS on HALO RP-Amide
AN004 Vanillins on HALO C18
AN005 Vanillins on HALO Phenyl-Hexyl
AN006 Anilines on HALO C18
AN007 Anilines on HALO RP-Amide
AN008 Basic Drugs on HALO PFP
AN009 Neutral Aromatics on HALO PFP, C18 and Phenyl-Hexyl
AN010 Phthalate Esters on HALO RP-Amide
AN011 Aromatic Nitro compounds on HALO PFP and Phenyl-Hexyl
AN012 Fibrates (cholesterol lowering drugs) on HALO PFP
AN013 Fibrates (cholesterol lowering drugs) on HALO RP-Amide
AN014 Fibrates (cholesterol lowering drugs) on HALO C18
AN015 Dinitrotoluenes on HALO RP-Amide
AN016 Dinitrotoluenes on HALO PFP
AN017 Sulfonyl Urea Drugs on HALO C18
AN018 Sulfonyl Urea Drugs on HALO Phenyl-Hexyl
AN019 Sulfonyl Urea Drugs on HALO PFP
AN020 Triazine Pesticides on HALO C18
AN021 Statin Drugs on HALO C8
AN022 Statin Drugs on HALO Phenyl-Hexyl
AN023 Steroids on HALO C18 and PFP
AN024 Benzodiazepines on HALO Phenyl-Hexyl, C18, and PFP Phases
AN025 NSAIDs on HALO ES-CN Phase
AN026 Amphenicols on HALO Phenyl-Hexyl Phase
AN027 Carbamate Pesticides on HALO ES-CN Phase
AN028 Carbamate Pesticides on HALO C18 Phase
AN029 Peptides and Small Proteins on HALO Peptide ES-C18
AN030 Anti-ulcer Drugs on HALO Penta-HILIC
AN031 Fluoroquinolone Drugs on HALO Phenyl-Hexyl Phase
AN032 Antidepressants on HALO Penta-HILIC Stationary Phase
AN033 Cephalosporins on HALO ES-CN
AN034 Penicillins on HALO ES-CN
AN035 Penicillins on HALO Phenyl-Hexyl
AN036 Phenylurea Herbicides on HALO-5 C18 and a Competitive 3um Totally Porous C18 Phase
AN037 Antibiotic and Antifungal Drugs on HALO RP-Amide
AN038 Diosmin and Hesperidin Flavonoids on HALO Phenyl-Hexyl and HALO RP-Amide
AN039 Anesthetics on HALO 2 Penta-HILIC
AN040 Steroids on HALO 2 PFP
AN041 Benzodiazepines on HALO 2 Phenyl-Hexyl
AN042 Steroids on HALO 90A Biphenyl
AN043 Pesticides on HALO 90A Biphenyl
AN044 IgG2 on HALO 1000A C4, ES-C18, and Diphenyl

Articles

LC-GC Europe Magazine: Joseph Jack Kirkland - the HPLC Particle Pioneer