HALO
Hyper-fast, super rugged HPLC columns
The fused-core* particle technology was specially
developed to produce hyper-fast chromatographic separation while avoiding
the uncertainties associated with UHPLC. The father of the technique is dr.
Jack Kirkland, widely regarded as one of the "founders" of HPLC.
* or core-shell, or
superficially-porous, or semi-porous, or pellicular
BACK PRESSURE - significantly less compared to UHPLC
columns
CONVENTIONAL HPLC EQUIPMENT can be used with near-UHPLC performance
2um INLET FRIT eliminates frit plugging problem
FAST SEPARATION at high flow rates maintaining the resolving power
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HALO 2.7um Specifications
- Ultra-pure type "B" silica support
- 1.7um solid core with 0.5um porous silica layer fused to the surface
- 150 m2/g surface
- 90A or 1000A (C4) pore size
- Monomeric bonding chemistry
- pH range: 2 to 9
- Max. pressure: 600 bar
Chemistries
C4 ...
The
world’s first 2.7-micron superficially porous silica particles with 1000
Angstrom pores for the separation of very large molecules such as monoclonal
antibodies, large proteins, synthetic polymers,
etc.
C8 ...
Separations are due primarily to hydrophobic interactions and differences in
hydrophobicity among analytes. Excellent for a broad range of analytes.
C18 ... Separations are due
primarily to hydrophobic interactions and differences in hydrophobicity
among analytes. Excellent for a broad range of analytes.
Peptide ES-C18 ... Ideal for
ultrafast and high resolution separation of peptides and polypeptides (≤
15 kDa). Enhanced stability at low pH via sterically protected ligands up to
90 degrees C.
Phenyl-Hexyl ... Separations
occur due to hydrophobic and pi-pi interactions. Enhanced retention and
selectivity for aromatic and unsaturated analytes (especially those with
electron-withdrawing groups and halogens). Compatible with highly aqueous
mobile phases.
HILIC ... Can be used in both
HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS
analyses of basic analytes.
Penta-HILIC ... Provides
superior retention to silica HILIC and amide HILIC phases for acids and
zwitterionic analytes
PFP ... Separations take place
due to multiple types and degrees of analyte interactions including
hydrophobic, pi-pi, dipole-dipole, and hydrogen bonding. Can be used in
HILIC mode (with more than 80% acetonitrile in mobile phase). Enhanced
selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
RP-Amide ... Retention and
selectivity due to hydrophobic and hydrogen bonding interactions. Less
retentive than C18 and C8, except for analytes with proton-donor groups.
Compatible with 100% aqueous mobile phases.
Peptide ES-CN ... Provides strong
dipole-dipole interactions with analytes and weak hydrophobic interactions.
Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid
equilibration with low bleed for high throughput LC-MS applications. Extra
stable bonding with sterically protected ligand provides improved stability
and performance compared to conventional CN phases, especially at low pH
(1-4).
HALO 3.4um Specifications
- Ultra-pure type "B" silica support
- 3.0um solid core with 0.2um porous silica layer fused to the surface
- 400A pore size
Chemistries
Protein C4
... Dimethylbutylsilane, exhaustively endcapped and enhanced for high
temperature stability.
Protein ES-C18 ...
Reversed-phase wide-pore packing for separating mid-high molecular weight
compounds such as proteins and polypeptides < 500 kDa
Protein Diphenyl ... Increased
Resolution compared to FPP 300A Diphenyl
- Ultra-pure type "B" silica support
- 3.8um solid core with 0.6um porous silica layer fused to the surface
- 90 m2/g surface (equivalent to 150-200m2/g totally porous particles)
- 90A pore size
- Monomeric bonding chemistry
- pH range: 2 to 9
- Max. pressure: 600 bar
Chemistries
C8 ...
Separations are due primarily to hydrophobic interactions and differences in
hydrophobicity among analytes. Excellent for a broad range of analytes.
C18 ... Separations are due
primarily to hydrophobic interactions and differences in hydrophobicity
among analytes. Excellent for a broad range of analytes.
Peptide ES-C18 ... Ideal for
ultrafast and high resolution separation of peptides and polypeptides (≤
15 kDa). Enhanced stability at low pH via sterically protected ligands up to
90 degrees C.
Phenyl-Hexyl ... Separations
occur due to hydrophobic and pi-pi interactions. Enhanced retention and
selectivity for aromatic and unsaturated analytes (especially those with
electron-withdrawing groups and halogens).
HILIC ... Can be used in both
HILIC and normal-phase modes. Enhanced sensitivity and peak shape for LC-MS
analyses of basic analytes.
Penta-HILIC ... Provides
superior retention to silica HILIC and amide HILIC phases for acids and
zwitterionic analytes
PFP ... Separations take place
due to multiple types and degrees of analyte interactions including
hydrophobic, pi-pi, dipole-dipole, and hydrogen bonding. Can be used in
HILIC mode (with more than 80% acetonitrile in mobile phase). Enhanced
selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
RP-Amide ... Retention and
selectivity due to hydrophobic and hydrogen bonding interactions. Less
retentive than C18 and C8, except for analytes with proton-donor groups.
Compatible with 100% aqueous mobile phases.
Peptide ES-CN ... Provides strong
dipole-dipole interactions with analytes and weak hydrophobic interactions.
Suitable for use in reversed-phase, HILIC, and normal-phase modes. Rapid
equilibration with low bleed for high throughput LC-MS applications. Extra
stable bonding with sterically protected ligand provides improved stability
and performance compared to conventional CN phases, especially at low pH
(1-4).
| Packing Description | Bonded Phase | Retention Mode | Target Analytes |
| HALO Peptide ES-C18 | Peptide ES-C18 (diisobutyloctadecylsilane) |
Hydrophobic interactions | A stable, reversed-phase packing used for separating peptides and polypeptides < 20 kDa |
| HALO Peptide ES-CN | Peptide ES-CN (diisopropylcyanopropylsilane) |
Hydrophobic interactions | An alternative stable, reversed-phase packing that can be used for separating peptides and polypeptides < 20 kDa |
| HALO Protein C4 | Protein C4 (dimethylbutylsilane) |
Hydrophobic interactions | A wide-pore stable, reversed-phase packing used for separating high molecular weight compounds such as proteins and polypeptides < 500 kDa |
| HALO Protein ES-C18 | Protein ES-C18 (diisobutyloctadecylsilane) |
Hydrophobic interactions | An alternative stable, reversed-phase wide-pore packing for separating mid-high molecular weight compounds such as proteins and polypeptides < 500 kDa |
| HALO Protein Diphenyl | Protein 400A Diphenyl (Diphenylmethylsilane) |
Hydrophobic interactions | Designed on a 3.4um particle providing the speed, ruggedness and resolution desired for quick release assays and flexible to method conditions to express necessary critical quality attributes |
| HALO Glycan | Glycan proprietary penta-hydroxy-ligand |
Hydrophilic interaction polyhydroxy bonded phase w. adsorbed aqueous layer |
Ideal for separation of highly polar oligosaccharides and particularly for protein-linked glycans and glycopeptides |
| Packing Description | Endcapped | Particle, um | Pore, A | Max P (bar) | Max T (°C) | pH | Carbon, % |
| HALO Peptide ES-C18 | No | 2 / 2.7 / 5 | 160 | 600 | 90 | 1 - 8 | 4.6 / 4.0 |
| HALO Peptide ES-CN | Yes | 2.7 / 5 | 160 | 600 | 90 | 1 - 8 | 2.2 / 1.5 |
| HALO C30 | Yes | 2.7 | 160 | 600 | 40 | 2 - 9 | 4.5 |
| HALO Protein C4 | Yes | 3.4 | 400 | 600 | 90 | 2 - 9 | 0.4 |
| HALO Protein ES-C18 | Yes | 3.4 | 400 | 600 | 90 | 1 - 8 | 1.0 |
| HALO Protein Diphenyl | Yes | 3.4 | 400 | 600 | 90 | 2 - 9 | --- |
| HALO Glycan | No | 2.7 | 90 | 600 | 65 | 2 - 9 | 3.2 |
| HALO C4 1000 | Yes | 2.7 | 1000 | 600 | 40/90 | 2 - 9 | 0.6 |
| HALO ES-C18 1000 | Yes | 2.7 | 1000 | 600 | 40/90 | 1 - 8 | 1.4 |
| HALO Diphenyl 1000 | Yes | 2.7 | 1000 | 600 | 40/90 | 2 - 9 | 1.0 |
News
HALO C30 columns:
best for fat/water soluble vitamins, carotenoids, lipids, steroids. 100 %
aqueous compatibility.
HALO 160A C30 Columns
Catalogs
HALO Catalog
HALO Bioclass Columns
HALO Applications Collection
HALO Method Conversion
Guidebook
HALO Guidebook on Reverse Phase
Chemistries
HALO Small Molecule
Method Development Guide
HALO Biotherapeutic
Method Development Guide
Brochures
HALO HILIC Columns
HALO HILIC Column Care
HALO Penta-HILIC Columns
HALO PFP Columns
HALO PFP Column Care
HALO PhenylHexyl Columns
HALO RP-Amide Columns
HALO Guard Cartridges
HALO Peptide ES-C18
HALO Protein and Peptide
Columns
HALO ES-CN flyer
HALO 5um Columns
HALO-5 C8 Column Care
HALO-5 ES-CN Column Care
HALO-5 PhenylHexyl Column Care
HALO-5 HILIC Column Care
HALO-5 Penta-HILIC Column Care
HALO-5 RP-Amide Column Care
HALO 1000A C4 Columns
HALO 1000A Diphenyl Columns
HALO 2um ES-C18 Peptide Columns
HALO 2um Columns
HALO 160A Phenyl-Hexyl Columns
HALO 90A Biphenyl Columns
HALO 90A AQ-C18 Columns
HALO 400A Diphenyl Columns
Posters, Presentations
New Fused-Core Particles for Very
Fast HPLC Separations (Article)
New Fused-Core Particles for Very
Fast HPLC Separations (Poster)
Nano-Capillary HPLC with Fused-core Particles
for Proteomics Applications
Analysis of Lipids by HPLC (HALO C8)
Converting Conventional RP-HPLC
Separations to Ultra-Fast Separations
Bridge the Gap between UHPLC and HPLC
Modifying Agilent 1100 HPLC Systems to
Achieve "UHPLC-like" Performance
HALO HILIC 2.7 and 5um Column Comparison
HALO Penta-HILIC 2.7 and 5um Column Comparison
HALO 1000As: Selectivity
Manipulation for LC/MS Analysis of Protein Variants
HALO 90A AQ-C18: LC/MS
Method for the Analysis of Guanine Deaminase
Applications
AN001 NSAIDS on HALO C18
AN002 NSAIDS on HALO C8
AN003 NSAIDS on HALO
RP-Amide
AN004 Vanillins on HALO
C18
AN005 Vanillins
on HALO Phenyl-Hexyl
AN006 Anilines on HALO C18
AN007 Anilines on HALO
RP-Amide
AN008 Basic Drugs on
HALO PFP
AN009 Neutral Aromatics on HALO PFP, C18 and Phenyl-Hexyl
AN010 Phthalate
Esters on HALO RP-Amide
AN011 Aromatic Nitro compounds on HALO PFP and Phenyl-Hexyl
AN012 Fibrates (cholesterol lowering drugs) on HALO PFP
AN013 Fibrates (cholesterol lowering drugs) on HALO RP-Amide
AN014 Fibrates (cholesterol lowering drugs) on HALO C18
AN015
Dinitrotoluenes on HALO RP-Amide
AN016 Dinitrotoluenes on HALO PFP
AN017 Sulfonyl Urea
Drugs on HALO C18
AN018
Sulfonyl Urea Drugs on HALO Phenyl-Hexyl
AN019 Sulfonyl Urea Drugs on HALO PFP
AN020 Triazine
Pesticides on HALO C18
AN021 Statin Drugs on HALO C8
AN022 Statin Drugs on HALO Phenyl-Hexyl
AN023 Steroids on HALO C18 and PFP
AN024 Benzodiazepines on HALO Phenyl-Hexyl, C18, and PFP Phases
AN025 NSAIDs on HALO
ES-CN Phase
AN026
Amphenicols on HALO Phenyl-Hexyl Phase
AN027
Carbamate Pesticides on HALO ES-CN Phase
AN028
Carbamate Pesticides on HALO C18 Phase
AN029 Peptides and
Small Proteins on HALO Peptide ES-C18
AN030 Anti-ulcer Drugs on HALO Penta-HILIC
AN031
Fluoroquinolone Drugs on HALO Phenyl-Hexyl Phase
AN032 Antidepressants on HALO Penta-HILIC Stationary Phase
AN033 Cephalosporins on HALO ES-CN
AN034 Penicillins on HALO ES-CN
AN035 Penicillins on HALO Phenyl-Hexyl
AN036 Phenylurea
Herbicides on HALO-5 C18 and a Competitive 3um Totally Porous C18 Phase
AN037 Antibiotic and Antifungal Drugs on HALO RP-Amide
AN038 Diosmin and Hesperidin Flavonoids
on HALO Phenyl-Hexyl and HALO RP-Amide
AN039
Anesthetics
on HALO 2 Penta-HILIC
AN040 Steroids
on HALO 2 PFP
AN041
Benzodiazepines
on HALO 2 Phenyl-Hexyl
AN042 Steroids on
HALO 90A Biphenyl
AN043
Pesticides on HALO 90A Biphenyl
AN044 IgG2 on HALO 1000A
C4, ES-C18, and Diphenyl
AN198 High speed LC-MS/MS
Separation of Mycotoxins on 2um PFP
Articles
LC-GC Europe
Magazine:
Joseph Jack Kirkland - the HPLC Particle Pioneer
Understanding and Overcoming
Separation Challenges in the Biological Drug Development Process
