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News

HALO PAH, PFAS phases

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Promotion

ACE Method Development Kits

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Events

33rd ISC (Budapest, Hungary)

21-25 September, 2020

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... the original fused-core particles

Hyper-fast, super rugged (U)HPLC columns

The fused-core* particle technology was specially developed to produce hyper-fast chromatographic separation while avoiding the uncertainties associated with UHPLC. The father of the technique is dr. Jack Kirkland, widely regarded as one of the "founders" of HPLC.
     * or core-shell, or superficially-porous, or semi-porous, or pellicular

Fused-Core Particle

BACK PRESSURE - significantly less compared to UHPLC columns
CONVENTIONAL HPLC EQUIPMENT can be used with near-UHPLC performance
2um INLET FRIT eliminates frit plugging problem
FAST SEPARATION at high flow rates maintaining the resolving power

 

HALO columns for small molecules

HALO columns for small molecules 

Phase  Particle (um) Pore (A)  Carbon load (%)  pH limit 

C8
2
2.7
5

90
4.8
5.4
3.7

2 - 9

C18
2
2.7
5

90
7.2
7.7
5.4

2 - 9

C18 (AQ)
2
2.7

90

6.5 / 6.7

2 - 9
C30 2.7
160 4.5
2 - 9
Biphenyl 2.7
90 7.0
2 - 9

Phenyl-Hexyl
2
2.7
5

90
6.3
7.1
5.2

2 - 9

RP-Amide
2
2.7
5

90
7.3
8.2
5.1

2 - 9

PFP
2
2.7
5

90
5.3
5.5
3.9

2 - 8
PAH 2.7
90 9.9
2 - 9
PFAS 2.7
90 9.9
2 - 9

ES-CN
2
2.7
5

90
3.4
3.5
2.5

1 - 8

HILIC
2
2.7
5

90

unbonded

1 - 8

Penta-HILIC
2
2.7
5

90
2.8
3.2
2.1

2 - 9
 

 

Chemistries

HALO C8 phase 
Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes.
HALO C18 phase 
Separations are due primarily to hydrophobic interactions and differences in hydrophobicity among analytes. Excellent for a broad range of analytes.
HALO C18aq phase 
 Resistant to dewetting and compatible with 100% aqueous mobile phases.
Retains polar molecules more than classic C18 phases.
     
HALO C30 phase 
High shape selectivity for hydrophobic, long-chain, structurally related isomers.
100% aqueous compatibility.
HALO Biphenyl phase 
Separation of polar compounds with a combination of hydrophobic, aromatic, and polar selectivities.
HALO Phenyl-Hexyl phase 
Separations occur due to hydrophobic and pi-pi interactions. Enhanced retention and selectivity for aromatic and unsaturated analytes (especially those with electron-withdrawing groups and halogens). Compatible with highly aqueous mobile phases.
     
HALO RP-Amide phase 
Retention and selectivity due to hydrophobic and hydrogen bonding interactions. Less retentive than C18 and C8, except for analytes with proton-donor groups. Compatible with 100% aqueous mobile phases.
HALO PFP phase 
Separations take place due to multiple types and degrees of analyte interactions including hydrophobic, pi-pi, dipole-dipole, and hydrogen bonding. Can be used in HILIC mode (with more than 80% acetonitrile in mobile phase). Enhanced selectivity for stereoisomers. Compatible with highly aqueous mobile phases.
HALO PAH phase 
This trifunctional, un-endcapped C18 bonded phase has been specifically designed to provide fast, efficient, selective separations of PAH compounds.
     
HALO ES-CN phase 
Moderately polar phase for alternate selectivity.
Steric-protected silane for low pH stability.
Fully end-capped for neutral pH applications.
HALO HILIC phase 
Can be used in both HILIC and normal-phase modes.
Enhanced sensitivity and peak shape for LC-MS analyses of basic analytes.
HALO penta-HILIC phase 
Provides superior retention to silica HILIC and amide HILIC phases for acids and zwitterionic analytes.

 

 

HALO BioClass columns

HALO BioClass columns 

Phase  Particle (um) Pore (A)  Carbon load (%)  pH limit 
C4 3.4 400 0.4 2 - 9
C4 2.7 1000 0.6 2 - 9

ES-C18
2
2.7
5

160
4.0
4.6
4.0

1 - 8
ES-C18  3.4 400 1.0 1 - 8
ES-C18 2.7 1000 1.4 1 - 8
Diphenyl 2.7 1000 0.9 2 - 9
Phenyl-Hexyl 2.7 160 4.7 2 - 9
ES-CN  2.7
5
160 2.2
1.5
1 - 8
Glycan 2.7 90 3.2 2 - 9

 

Chemistries

HALO BioClass C4 HALO BioClass ES-C18 HALO BioClass Diphenyl
     
HALO BioClass Phenyl-Hexyl HALO BioClass ES-CN HALO BioClass Glycan

HALO Guard system
AMT use the Optimize Technologies EXP Direct Connect Holder

HALO guard system

KEY FEATURES
- Hand-tight cartridge replacement
- Hardware rated to 20,000+ psi (1,400+ bar)
- 1.4uL Swept Volume (excluding packed bed)
- Auto-adjusting ZDV column connection
- Hardened stainless steel end cap eliminates galling
- Features Free-Turn architecture
- For use with EXP Trap and Guard Cartridges
- Fittings included

 

 

 

Downloads

News

HALO PAH columns: this trifunctional C18 bonded phase provides fast, efficient, selective separation of PAH compounds.
HALO 90A PAH Columns

HALO PFAS columns: developed for the analysis of short-chain and long-chain PFAS (per- and polyfluoroalkyl substances)
HALO 90A PFAS Columns


Catalogs, Guides

HALO General Catalog

HALO Enviro-class Catalog

HALO Method Conversion Guidebook
HALO Guidebook on Reverse Phase Chemistries
HALO Small Molecule Method Development Guide

HALO Bioclass Columns
HALO Bioanalysis Guidebook
HALO Biotherapeutic Method Development Guide


 Brochures, Care-and-Use Sheets

Phase Brochure Care-and-Use
C4 HALO 1000A C4 Columns HALO 400A C4 Column Care
HALO 1000A C4 Column Care
C8   HALO-2 90A C8 Column Care
HALO-2.7 90A C8 Column Care
HALO-5 90A C8 Column Care
C18   HALO-2 90A C18 Column Care
HALO-2.7 90A C18 Column Care
HALO-5 90A C18 Column Care
ES-C18 HALO-2 160A Peptide ES-C18 Columns
Eternity PH HALO-2.7 160A Peptide ES-C18 Columns
HALO Protein and Peptide Columns
Eternity PH HALO-2.7 160A Peptide ES-C18 Column Care
Eternity PH HALO-5 160A Peptide ES-C18 Column Care
Eternity PH HALO-3.4 400A Protein ES-C18 Column Care
Eternity PH HALO-2.7 1000A ES-C18 Column Care
C18(AQ) HALO 90A AQ-C18 Columns HALO-2 90A C18(AQ) Column Care
HALO-2.7 90A C18(AQ) Column Care
HALO-5 90A C18(AQ) Column Care
C30 HALO 160A C30 Columns Eternity PH HALO-2.7 160A C30 Column Care
Biphenyl HALO 90A Biphenyl Columns HALO-2 90A Biphenyl Column Care
HALO-2.7 90A Biphenyl Column Care
HALO-5 90A Biphenyl Column Care
Diphenyl HALO 400A Diphenyl Columns
HALO 1000A Diphenyl Columns
HALO-2.7 1000A Diphenyl Column Care
Phenyl-Hexyl HALO 90A Phenyl-Hexyl Columns
HALO 160A Phenyl-Hexyl Columns
HALO-2 90A Phenyl-Hexyl Column Care
HALO-2.7 90A Phenyl-Hexyl Column Care
HALO-5 90A Phenyl-Hexyl Column Care
HALO-2.7 160A Phenyl-Hexyl Column Care
RP-Amide HALO 90A RP-Amide Columns HALO-2 90A RP-Amide Column Care
HALO-2.7 90A RP-Amide Column Care
HALO-5 90A RP-Amide Column Care
PFP HALO 90A PFP Columns HALO-2 90A PFP Column Care
HALO-2.7 90A PFP Column Care
HALO-5 90A PFP Column Care
PAH HALO 90A PAH Columns HALO-2.7 90A PAH Column Care
PFAS HALO 90A PFAS Columns HALO-2.7 90A PFAS Column Care
HALO-2.7 90A PFAS Delay Column Care
ES-CN   HALO-2 90A ES-CN Column Care
HALO-2.7 90A ES-CN Column Care
HALO-5 90A ES-CN Column Care
HALO-2.7 160A Peptide ES-CN Column Care
HALO-5 160A Peptide ES-CN Column Care
HILIC HALO 90A HILIC Columns HALO-2 90A HILIC Column Care
HALO-2.7 90A HILIC Column Care
HALO-5 90A HILIC Column Care
penta-HILIC HALO 90A Penta-HILIC Columns HALO-2 90A penta-HILIC Column Care
HALO-2.7 90A penta-HILIC Column Care
HALO-5 90A penta-HILIC Column Care
Glycan (link) HALO-2.7 90A Glycan Column Care

 
HALO 2um Columns
HALO 5um Columns
HALO Guard Cartridges


 Posters, Presentations

New Fused-Core Particles for Very Fast HPLC Separations (Article)
New Fused-Core Particles for Very Fast HPLC Separations (Poster)
Take Your UHPLC and HPLC Methods to the Next Level of Speed and Resolution
Nano-Capillary HPLC with Fused-core Particles for Proteomics Applications
Analysis of Lipids by HPLC (HALO C8)
Converting Conventional RP-HPLC Separations to Ultra-Fast Separations

Bridge the Gap between UHPLC and HPLC
Modifying Agilent 1100 HPLC Systems to Achieve "UHPLC-like" Performance

HALO HILIC 2.7 and 5um Column Comparison
HALO Penta-HILIC 2.7 and 5um Column Comparison
HALO 1000As: Selectivity Manipulation for LC/MS Analysis of Protein Variants
HALO 90A AQ-C18: LC/MS Method for the Analysis of Guanine Deaminase

 

Applications

HALO Applications Collection

Anti-diabetic Drugs
Anti-ulcer Drugs
Antibiotics
Anticoagulants
Antidepressants
Antimicrobial Drugs (Amphenicols)
Aromatic Amines
Aromatic Compounds, PAHs
Basic Drugs
Benzodiazepines
Beta Blockers
Cannabinoids
Carbamate Insecticides
Carbonyl Compounds (DNPH)
Carotenoids
Cephalosporins
Decongestants
Dietary Supplements, Edible Oils
Environmental
Environmental - PFAS
Explosives
Flavonoids
Herbicides
Hormones
Hypolipidemic Drugs
Mobile Phase Additives (mAb)
Monoclonal Antibodies
Mycotoxins
Natural Products
NSAIDs
Nucleobases
Pain Management Drugs
Peptides
Pesticides
Phenolic Acids
Phthalates
Organic Acids, Bile Acids
Proteins
Psychiatric Drugs
Statins
Steroids
Vanillins
Vitamins
Xanthines


Articles

LC-GC Europe Magazine: Joseph Jack Kirkland - the HPLC Particle Pioneer
Understanding and Overcoming Separation Challenges in the Biological Drug Development Process
Environmental Analyses of Harmful Chemicals

Technical Reports

LC-MS/MS methods for the analysis of short-chain and long-chain PFAS

 

Useful links

HALO Application Notes

Find a column part number