SynChropak

 

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A new approach to biomarker discovery

Phases made by a world-renowned bonding chemistry

Eprogen, Inc. produce NPS (Non Porous Silica), and porous phases based on the SynChropak bonding chemistry. Listed below are these columns (packed by HiChrom).

MICRA / SynChropak porous phases

Phase Functional Group Micra Particle, um Pore, A
RP-1 C1 Silver 6 300
SCD-100 C2 Gold 5 100
RP-4 C4 (polymeric) Silver 6 300
RP-8 C8 (monomeric) Silver 6 300
Q300 SAX Silver 6 300
S300 SCX Silver 6 300
AX100 WAX Gold 6 100
AX300 WAX Silver 6 300
CM100 WCX Gold 6 100
CM300 WCX Silver 6 300

 

 

All Hichrom manufactured SynChropak columns use standard compression fittings.

Guard cartridge system
GUARD CARTRIDGE SYSTEM
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MICRA-Gold SCD-100 is a short alkyl chain ligand bonded phase on 5µm, 100Å, spherical silica with state of the art base-deactivation. Excellent peak shapes are obtained for many drug mixtures without the addition of silanol suppressing agents. MICRA-Gold SCD-100 is ideal for the analysis of positively charged molecules, as well as neutral and acidic molecules.

MICRA-Silver RP-4 is a polymerically bonded C4 phase on 6µm, 300Å, spheroidal silica. C4 is a popular choice for protein and peptide separations especially for large hydrophobic solutes. The 300Å pore size is optimal for the loading and high resolution of peptides. C4 ligands are often used for protein separations due to their excellent recovery.

MICRA-Silver RP-8 is a monomerically bonded C8 phase on 6µm, 300Å, spheroidal silica. As the carbon chain length has little effect on selectivity in reversed phase chromatography of peptides and proteins, the RP-8 Silver column is suited for separating smaller proteins and peptides, especially those of a less hydrophobic nature.

MICRA-Silver Q300 is a quaternized, crosslinked polyethyleneimine phase on 6µm, 300Å, spheroidal silica. This strong anion exchanger is compatible with aqueous and many organic solvents in the pH range of 2-8. Ionization of this support has no pH dependence. This is an excellent column for the rapid separation of proteins and enzymes.

MICRA-Silver S300 has a polyamide coating containing sulfopropyl functional groups, bonded to a 6µm, 300Å, spheroidal silica. This strong cation exchanger is compatible with aqueous and many organic solvents in the pH range of 2-8. Ionization of the functional groups on this bonded phase is constant above pH 3. This column is a good choice for separations of basic proteins.

MICRA-Gold AX100 is a crosslinked polyethyleneimine phase on 5µm, 100Å, spherical silica. This weak anion exchanger is compatible with aqueous and many organic solvents in the pH range of 2-8. Selectivity can be altered by mobile phase composition, and pH affects ionization of both the support and the solute. Similar to AX300, this 100Å support provides a high surface area for the separation of small molecules. AX100 provides excellent separations of nucleotides, acidic molecules and oligonucleotides of up to 30 residues.

MICRA-Silver AX300 is a crosslinked polyethyleneimine phase on 6µm, 300Å, spheroidal silica. This weak anion exchanger is compatible with aqueous and many organic solvents in the pH range of 2-8. Selectivity can be altered by mobile phase composition, and pH affects ionization of both the support and the solute. AX300 permits analysis of proteins up to molecular weights of 200,000 daltons. AX300 has excellent recoveries and loading capacity. For example, 22mg ovalbumin can be loaded onto a 250x4.6 column with no overloading effects.

MICRA-Gold CM100 has a polyamide coating containing carboxymethyl groups and is bonded to a 5µm, 100Å, spherical silica. This weak cation exchanger is compatible with aqueous and many organic solvents in the pH range of 2-8. Selectivity can be altered by mobile phase salt composition, and pH affects ionization of both the support and the solute. This phase is a superb choice for the separation of small cationic compounds such as catecholamines.

MICRA-Silver CM300 has a polyamide coating containing carboxymethyl groups and is bonded to a 6µm, 300Å, spheroidal silica. This weak cation exchanger is compatible with aqueous and many organic solvents in the pH range of 2-8. Selectivity can be altered by mobile phase salt composition, and pH affects ionization of both the support and the solute. CM300 permits analysis of proteins up to molecular weights of 200,000 daltons and exhibits excellent recoveries as well as high loading capacity.

 

 

 

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Catalogs

MICRA HPLC Catalog