SIELC Technologies
Primesep 100 and Obelisc R / N - probably the most universal columns ever made
SIELC Technologies manufacture the Primesep HPLC columns, specifically designed for mixed-mode separations. Primesep columns are capable of separating a tremendous range of compounds by different separation modes based only upon mobile phase selection. These columns were designed with the understanding that proper inclusion, not elimination, of secondary interaction is a powerful tool in selectivity control, column stability, and separation reproducibility.
The Obelisc columns are the first commercially available columns with Liquid Separation Cell technology. Obelisc R, with reversed-phase characteristics, and Obelisc N, with normal-phase characteristics, separate polar and non-polar compounds using multiple separation mechanisms. This duo replaces multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. Method development is simplified by using only two Obelisc columns and a few simple mass spec or low UV (<220 nm) compatible mobile phases.
The separation of peptides and proteins by Promix columns is based on a combination of two interactions - hydrophobic and ionic. This is possible due to a new type of separation media - a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support.
Primesep columns
|
Acidic Primesep phases (mixed-mode
cation exchange columns) |
Analysis of ... |
|
Primesep A (ionized in all
working pH) the strongest acidic phase |
weak bases, amino acids, metals |
|
Primesep 100 (transition pH =
1) The most versatile column of the family strong acidic phase |
weak / medium bases, amino acids, metals |
|
Primesep P (transition pH = 1)
Separates aromatics with pi-pi interaction medium acidic phase |
weak / medium aromatic bases |
|
Primesep 200 (transition pH =
2) Separates isomers and structurally-related compounds weak acidic phase |
strong bases, dibases |
|
Primesep 500 (transition pH =
5) weakest acidic phase |
strong bases, dibases, polybases |
In order to get retention by cation-exchange mechanism on Primesep columns, the pH of the mobile phase should be selected close to, or above, the transition value of embedded acidic groups. Below the transition pH value, the column behaves as a regular reverse-phase column with an embedded non-ionized polar group and can be used in 100% aqueous mobile phase ("AQ" type column).
|
Basic Primesep phases (mixed-mode
anion exchange columns) |
Analysis of ... |
|
Primesep B (pH range 1.5 - 4) strong basic phase |
acids, bases, neutrals (add trifluoroacetic, phosphoric, perchloric or formic acid to the mobile phase) |
|
Primesep B2 (pH range 1.5 - 7) weak basic phase |
acids, bases, neutrals (add ammonium acetate to the mobile phase) |
|
Primesep B4 (pH range 2 - 4.5) embedded basic ion-pairing groups |
acids, bases, neutrals, hydrophobic bases, surfactants |
|
Primesep D (pH range 1.5 - 4) designed for direct plasma injection |
hydrophobic, acidic components; proteins and peptides elute as a sharp peak in void or pre-void time |
|
Primesep SB (pH range 1.5 - 5) strong basic phase |
acids, bases, neutrals, Zwitterionics, natural product extracts |
|
Primesep PB (pH range 2 - 5) embedded aromatic and basic ion-pairing groups |
acids, aromatics, neutrals |
|
Primesep AB (ionized in all
working pH) embedded acidic and basic ion-pairing groups |
strong acids and bases simultaneously |
|
Other Primesep phases |
Analysis of ... |
|
Primesep C (pH range 3 - 7)
Unique elution order of positively charged molecules embedded complex-forming groups |
amines, sulfonium, phosphonium and metal ions |
|
Primesep P embedded aromatic and acidic ion-pairing groups |
structural isomers of aromatics |
Obelisc columns
|
Multiple separation mode (RP, NP, HILIC, IE) |
Analysis of ... |
|
Obelisc N anions on the surface, separated from cations by hydrophilic chain |
sugars, amino acids, carboxylic acids, aromatic acids |
|
Obelisc R cations on the surface, separated from anions by hydrophobic chain |
polar drugs, amino acids, PAHs |
This duo replaces multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. Method development is simplified by using only two Obelisc columns and a few simple mobile phases.
Promix columns
|
Separation of peptides and proteins based on hydrophobic and ionic interactions |
Analysis of ... |
|
Promix SP 100A pore size |
small peptides under 1 kDa with pI value below 6 |
|
Promix AP 100A and 300A pore sizes |
small and medium size peptides under 3 kDa with pI value below 7 |
|
Promix MP 300A and 800A pore sizes |
medium size proteins and peptides (1 - 10 kDa) |
UHPLC Fittings
- Strong stainless steel thread allows many more
use-cycles than a one-piece PEEK design
- Finger-tight installation. PEEK tip installs permanently and cannot be
lost
- Pressure rating up to 14,000 psi (1000 bar)
- One-size-fits-most applications, including columns, valves, and instrument
ports
- Break-resistant - metal fitting can bend: but it won't break, and thus it
can be simply replaced with another fitting, saving the expensive column
- Improves heat transfer with the incoming capillary, when a column heater
is used
- PEEK and metal-capillary compatible
- Special capillary tube gripping mechanism allows strong holding effect of
the tube, even with very gentle tightening force
Picture (left side) - Fitting for quick-release applications (eg., column-to-instrument connections)
Picture (right side) - Fitting for semi-permanent applications (such as valve-to-instrument connections or pump-to-autosampler connections)
SIELC Newsletter
03/2010: SmartFittings for Smart LC Applications
News
Primesep S is a NP column for
sugars and other very polar neutral molecules
Separation of sugars on Primesep
S column
see link to
http://www.sielc.com/Products_Primesep_S.html
Catalogs
Primesep catalog
Obelisc catalog
Promix catalog
Brochures
Primesep - separation of ions
Primesep - solving
pharmaceutical problems
Posters, Presentations
A guide for HPLC
separation of small molecules - Primesep
Pharmaceutical analysis - Primesep
New mixed-mode approach -
Obelisc
New mixed-mode HILIC -
Obelisc
SIELC online Applications Guide
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