Evosphere monodisperse HPLC particles

more info ....


Your own HALO MDK: 3 for 2

more info ....


13th Balaton Symposium (Siofok, Hungary)

more info ....

... leadership in separations technology

One of the first commercially available  irregular silicas

Partisil is manufactured by Whatman International Ltd.  Partisil ODS-3 shows similar HPLC performance to Waters µBondapak.


On 29th February 2012, Hichrom acquired the Partisil & Partisphere HPLC column ranges from Whatman, a division of GE Healthcare (see announcement)

Partisil phases (Pore size: 80A)

Phase Functional Group Endcapped Particle, um Carbon load, %
Si - - 5, 10 -
C8 C8 Yes 5, 10 9
ODS C18 No 10 5
ODS-2 C18 Yes 10 16
ODS-3 C18 Yes 5, 10 10.5
PAC NH2-CN No 5, 10 16
SAX tetramethyl ammonium No 10 10
SCX sulphonic acid No 10 10


WCS = Whatman Compression Screw end fittings.
RAC-II = Rapid Analysis Chromatography columns, 2nd generation for faster analytical separations and reduced solvent consumption. Unique end fitting geometry eliminates unswept corners to maximize resolution and peak symmetry. Operates at low back pressure, even at high flowrates, prolonging column life. Connects easily to modern LC instrumentation. Available prepacked with PARTISIL 5um media.

Partisil Silica (5 and 10um)

Pure silica stationary phase for adsorption chromatography.  Partisil 10 is used particularly for routine separations for higher flow rates and lower back pressures.  Partisil 5 is used particularly for higher resolution and fast analysis.  These are the supports on which the Partisil bonded phases are based.

Partisil C8 (5 and 10um)

An end-capped C8 monomeric phase with at least 8.5% carbon load. Provides high efficiency and rapid mass transfer while maintaining excellent peak shape and stability over a range of aqueous mobile phase compositions. Recommended for ion pair chromatography.

Partisil ODS (10um)

A C18 phase with a 5% carbon load for both normal adsorption and reversed phase partitioning. Dual-mode operation for added selectivity with 50% residual silanols. Lightly loaded C18 packing is particularly effective for compounds having greater water solubility when used in the reversed phase mode. Creates a moderately polar surface, different from that of pure silica, in normal phase mode.

 Partisil ODS-2 (10um)

The high carbon load (16%) of this polymeric phase makes it the most nonpolar and, therefore, the most retentive of the reversed phases. An alternative to end-capped C18 where different elution order is desirable for optimum separation. High sample load capacity and 10um particle size are very suitable for preparative work.

 Partisil ODS-3 (5 and 10um)

 A C18 polymeric phase with a 10.5% carbon load. Medium of choice for improved speed, efficiency and resolution in applications requiring C18 phases. End-capped for deactivation of silanols to minimize the need for ion suppression or ion pairing agents. Used in a wide range of applications with optimal selectivity, including pharmaceuticals, natural products, food, biological and environmental pollutants.

Partisil PAC (5 and 10um)

 A polar amino-cyano (2:1 ratio) bonded phase with secondary amine groups for good thermal and chemical stability. Selectivity and rapid equilibrium allow a range of separation mechanisms to be used, including adsorption, reversed phase and weak anion exchange. Extremely fast equilibration across the entire range of solvents from heptane to water. The media of choice for carbohydrate separations.

Partisil SAX (5 and 10um)

A strong anion exchanger based on quaternary ammonium groups. Supplied in the H2PO4 form in methanol, Partisil 10 SAX has been widely reported in literature and is best known for the separation of nucleotides. Stable over pH range 1.5-7.5 when used in conjunction with a Solvecon mobile phase conditioning column. Obtains the highest anion exchange efficiencies and resolution. Applicable to separations of nucleic acids, organic acids and inorganic anions.

Partisil SCX (5 and 10um)

 Based on aromatic benzene sulfonic acid groups. Supplied in the ammonium form. Excellent for separation of nucleosides, amino acids, polyamines, drugs and other cationic species. Capable of being loaded with specific metallic cations for use in ligand exchange chromatography. Stable over pH range 1.5-7.0 when used in conjunction with a Solvecon mobile phase conditioning-column. Exceptionally stable Si-O-Si-C bond, both thermally and chemically.


All Hichrom manufactured Partisil columns use standard compression fittings.

Guard cartridge system
(JPEG file, opens in new window)