SIELC Technologies

SIELC Technologies manufacture the Primesep HPLC columns, specifically designed for mixed-mode separations. Primesep columns are capable of separating a tremendous range of compounds by different separation modes based only upon mobile phase selection. These columns were designed with the understanding that proper inclusion, not elimination, of secondary interaction is a powerful tool in selectivity control, column stability, and separation reproducibility.

The Obelisc columns are the first commercially available columns with Liquid Separation Cell technology. Obelisc R, with reversed-phase characteristics, and Obelisc N, with normal-phase characteristics, separate polar and non-polar compounds using multiple separation mechanisms. This duo replaces multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. Method development is simplified by using only two Obelisc columns and a few simple mass spec or low UV (<220 nm) compatible mobile phases.

The separation of peptides and proteins by Promix columns is based on a combination of two interactions - hydrophobic and ionic. This is possible due to a new type of separation media - a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support.

SHARC columns - they are the first commercially available columns with separation based primarily on hydrogen bonding.

Primesep columns (link)

Acidic Primesep phases (mixed-mode cation exchange columns)      with negatively charged functional groups	Analysis of ...
Primesep A (ionized in all working pH) the strongest acidic phase	weak bases, amino acids, metals link
Primesep 100 (transition pH = 1) The most versatile column of the family strong acidic phase	weak / medium bases, amino acids, metals link
Primesep 100 2.7um core-shell columns	weak / medium bases, amino acids, metals link
Primesep P (transition pH = 1) Separates aromatics with pi-pi interaction medium acidic phase	weak / medium aromatic bases link
Primesep 200 (transition pH = 2) Separates isomers and structurally-related compounds weak acidic phase	strong bases, dibases link
Primesep 500 (transition pH = 5) weakest acidic phase	strong bases, dibases, polybases link

Basic Primesep phases (mixed-mode anion exchange columns)      with positively charged functional groups	Analysis of ...
Primesep B (pH range 1.5 - 4) strong basic phase	acids, bases, neutrals (add trifluoroacetic, phosphoric, perchloric or formic acid to the mobile phase) link
Primesep B2 (pH range 1.5 - 7) weak basic phase	acids, bases, neutrals (add ammonium acetate to the mobile phase) link
Primesep B4 (pH range 2 - 4.5) embedded basic ion-pairing groups	acids, bases, neutrals, hydrophobic bases, surfactants link
Primesep D (pH range 1.5 - 4) designed for direct plasma injection	hydrophobic, acidic components; proteins and peptides elute as a sharp peak in void or pre-void time link
Primesep SB (pH range 1.5 - 5) strong basic phase	acids, bases, neutrals, Zwitterionics, natural product extracts link
Primesep SB 2.7um core-shell columns	acids, bases, neutrals, Zwitterionics, natural product extracts link
Primesep PB (pH range 2 - 5) embedded aromatic and basic ion-pairing groups	acids, aromatics, neutrals link
Primesep AB (ionized in all working pH) embedded acidic and basic ion-pairing groups	strong acids and bases simultaneously link

Other Primesep phases	Analysis of ...
Primesep AP silica-based amino column with hydrophilic properties	Separates acidic compounds using an anion-exchange mechanism, basic and neutral polar compounds using a HILIC mechanism. link
Primesep C (pH range 3 - 7) Unique elution order of positively charged molecules embedded complex-forming groups	amines, sulfonium, phosphonium and metal ions link
Primesep N normal-phase analytical column with embedded acidic groups	Features embedded acidic groups with a pKa of about 5. Enhances retention of basic compounds by cation-exchange mechanism at pH > 5. Separates polar compounds using the HILIC mechanism link
Primesep S normal-phase analytical column with embedded acidic ion-pairing groups	Suitable for the separation of common sugars and other neutral, very polar molecules. Retains and separates amino acids with different buffers at low concentration. Separates weak bases by ion-exchange mechanism, acids by ion-exclusion mechanism. link
Primesep S 2.7um core-shell columns	Suitable for the separation of common sugars and other neutral, very polar molecules. Retains and separates amino acids with different buffers at low concentration. Separates weak bases by ion-exchange mechanism, acids by ion-exclusion mechanism. link
Primesep S2 silica-based acidic column with hydrophilic properties	Separates basic compounds using a cation-exchange mechanism Separates basic and neutral polar compounds through a HILIC mechanism link
Primesep SB.M reverse-phase analytical column with strong embedded basic ion-pairing groups, containing a C18 carbon chain	Enhances retention of acidic compounds through anion-exchange mechanism Separates hydrophobic basic molecules using a combination of ion-exclusion and reverse-phase mechanism Retains neutral compounds by reverse-phase mechanism link

Obelisc columns (link)

Multiple separation mode (RP, NP, HILIC, IE)	Analysis of ...
Obelisc N anions on the surface, separated from cations by hydrophilic chain	sugars, amino acids, carboxylic acids, aromatic acids link
Obelisc R cations on the surface, separated from anions by hydrophobic chain	polar drugs, amino acids, PAHs link

This duo replaces multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. Method development is simplified by using only two Obelisc columns and a few simple mobile phases.

Promix columns (link)

Separation of peptides and proteins based on hydrophobic and ionic interactions	Analysis of ...
Promix SP 100A pore size	small peptides under 1 kDa with pI value below 6 link
Promix AP 100A and 300A pore sizes	small and medium size peptides under 3 kDa with pI value below 7 link
Promix MP 300A and 800A pore sizes	medium size proteins and peptides (1 - 10 kDa) link

SHARC columns (link)

BIST columns (link)

Bridge Ion Separation Technology (BIST) is a brand new LC separation technique developed by SIELC. The goal of BIST is simple – to achieve many separations that are traditionally difficult or impossible with any other HPLC columns currently on the market! In short, BIST is hassle-free complex chromatography. It provides an additional separation tool to a whole class of organic and inorganic charged molecules. BIST columns, simple mobile phases, and an array of applications provide a whole new continent on the chromatography map.

BIST – a New Mode of LC Separation

The Theory Behind BIST
BIST Applications
BIST Ionic Modifier Preparation

BIST Brochure

OMNI columns (link)

Traditional chromatography requires separate columns for reversed-phase, HILIC, ion-exchange, ion-exclusion, or normal phase separations. Chromni changes this paradigm by combining all interactions into a single, unified stationary phase.
SIELC Chromni – The All-in-One HPLC Column for Every Separation Mode!
With its dynamic and responsive surface chemistry, Chromni adapts to the mobile phase conditions — enabling precise control of selectivity and retention for a wide range of analytes. From polar to nonpolar compounds, acidic, basic, or neutral species, Chromni makes method development simpler, faster, and more efficient.

SIELC Chromni

LIPAK columns (link)

The new Lipak column utilizes mixed-mode chromatography, integrating reverse-phase functionality with a negatively charged group in its stationary phase.
This innovative design enables simultaneous retention and separation of both polar and nonpolar lipid species, effectively addressing the diverse chemical properties of lipid classes. By leveraging dual interaction modes, the Lipak column delivers superior resolution and reproducibility, even for complex lipid mixtures. This makes it an ideal solution for comprehensive lipidomics studies, ensuring precise identification and quantification of a broad range of lipid species while streamlining the analytical workflow.

Lipids by HPLC

OLIGO columns (link)

OligoMg is an oligonucleotide-specific column that employs the Bridge Ion Separation Technique (BIST). The column is dynamically loaded with a doubly charged positive ion (Mg²⁺), which, in the presence of an organic modifier in the mobile phase, creates a bridge between the negatively charged stationary phase and the negatively charged oligonucleotides.

SIELC OligoMg
Oligonucleotides by HPLC

PVP columns (link)

The PVP Column is a high-performance HPLC column specifically designed for accurate and reproducible analysis of polyvinylpyrrolidone (PVP) and related polymers across a wide molecular weight range. Built with a proprietary stationary phase optimized for polymer separations, it ensures sharp, symmetrical peaks with minimal adsorption—even for challenging amphiphilic compounds.

Polyvinylpyrrolidone by HPLC

PEI columns (link)

Polyethylenimine (PEI) has numerous industrial, medical, biological, and research applications. SIELC has developed a new methodology and a corresponding HPLC column offering a simple and reliable method for PEI quantitation in any liquid samples. This method is based on forming a PEI complex with Cu(II), which exhibits strong UV and visible light absorption maxima.

Polyethylenimine by HPLC

NEWCHROM columns (link)

	Analysis of ...
Newchrom A Newcrom A is a mixed-mode analytical column with negatively-charged acidic ion-pairing groups at the terminal end of long ligand chains.	–  acids by an ion-exclusion mechanism. – neutral compounds via a reverse-phase mechanism. – improved retention of weak basic compounds through a cation-exchange mechanism. – enhanced retention of multi-charged compounds. see link
Newchrom AH Newcrom AH is a mixed-mode analytical column with negatively-charged acidic ion-pairing groups at the terminal end of short ligand chains.	– weak basic compounds through a cation-exchange mechanism. – multi-charged compounds. – acids by an ion-exclusion mechanism. – neutral compounds via a reverse-phase mechanism. see link
Newchrom B Newcrom B is a mixed-mode analytical column with positively-charged basic ion-pairing groups at the terminal end of long ligand chains.	– multi-charged compounds. – bases using ion-exclusion mechanisms. – neutral compounds by reverse-phase mechanisms. see link
Newchrom BH Newcrom BH is a mixed-mode analytical column with positively-charged basic ion-pairing groups at the terminal end of short ligand chains.	– weak acidic compounds through an anion-exchange mechanism. – multi-charged compounds. – ases by an ion-exclusion mechanism. – neutral compounds via a reverse-phase mechanism. see link
Newcrom CN2 Designed for use in reversed-phase, normal-phase, and HILIC modes, making it an essential tool for method development and confirmation when alternate selectivity to traditional C18 or C8 columns is needed.	- polar and basic compounds. - Consistent and reproducible performance. - Minimal variation between batches. - Reduced solvent consumption due to lower retention in hydrophobic interactions compared to C18. - Best performance in pH range 1–7. see link
Newcrom R1 Special reverse-phase column with low silanol activity; stable at basic pH values, with a recommended pH range of 1 to 10.	Despite the fact that this stationary phase is stable at high pH, it is recommended to use an acidic mobile phase for column storage. A mobile phase composition with 0.1% phosphoric or formic acid in a 50/50 mixture of MeCN/H2O should be an ideal solution for both long and short-term storage. see link

SIELC Newcrom Brochure
SIELC Newcrom Applications