ProntoSIL
Original ProntoSIL and equivalency phases
ProntoSIL HPLC columns from Bischoff Chromatography are available in a wide variety of bonded phases and dimensions. In addition to more typical C8 and C18 phases, ProntoSIL columns are also available in hard to find bonded phases such as C1, amino, DIOL and C30.
| Phase | Functional group | Pore, A | Particle, um | Carbon, % | pH range | Use |
| Original ProntoSIL columns | ||||||
| ProntoSIL Si | Silica | 60/120/200/300 | 3/5/10 | --- | --- | ProntoSIL is the silica support of BISCHOFF Chromatography. It can be used in normal phase chromatography. |
| ProntoSIL C18 ace-EPS | C18 | 60/120/200/300 | 3/5/10 | 18.5 - 8.5 | 1 - 10 | ProntoSIL C18 ace-EPS belongs to the new group of stationary RP-supports with polar embedded groups. This packing offers a maximum of hydrophobicity combined with a maximum of polar selectivity. The silanophilic activity of the support is very low. Ultra strong basic compounds like amitriptyline can be eluted from the column in neutral pH values with excellent symmetrical peak shapes. |
| ProntoSIL C18 AQ | C18 | 120/200 | 3/5/10 | 14 - 9 | 3 - 8 | Used in aqueous mobile phases with an organic content below 10%. |
| ProntoSIL C18 AQ PLUS | C18 | 120 | 3/5/10 | 17 | 3 - 8 | ProntoSIL C18 AQ PLUS shows an enhanced stability at low pH’s down to pH 1. Also the packing shows excellent peak shapes in pure aqueous eluents but differs in shape selectivity compared to ProntoSIL C18 AQ. The application field for this support is mainly in combinatorial chemistry where the standard separation conditions are fast gradients from 0 – 100% organic and where the mobile phases include 0.1% TFA. |
| ProntoSIL C18 SH | C18 | 120 | 3/5/10 | 17 | 1 - 8 | This phase is fully endcapped. Due to the carbon load it shows an excellent shape selectivity and stability even at pH 1. |
| ProntoSIL C18 H | C18 | 60/120/200/300 | 3/5/10 | 18.5 - 7 | 3 - 8 | ProntoSIL C18 H is the standard C18 support of BISCHOFF Chromatography. It is applicable in a wide range of RP- chromatography. The packing is fully endcapped and shows all of the excellent properties a new generation stationary phase can offer. |
| ProntoSIL C8 ace-EPS | C8 | 120/300 | 3/5 | 12 - 9 | 3 - 8 | In comparison to ProntoSIL C18 ace-EPS, ProntoSIL C8 ace-EPS shows an even higher polar selectivity. Due to the shorter alkyl chain the influence of the polar group is increased. |
| ProntoSIL C8 SH | C8 | 60/120/200/300 | 3/5/10 | 12 - 4 | 1 - 8 | ProntoSIL C8 SH is a classical C8-type stationary phase; it is fully endcapped. Due to the bonding technology it shows an excellent shape selectivity and stability even at pH 1. The 5um support is also available in 60A. This packing shows an enhanced selectivity comparable to Lichrospher Select B from Merck. |
| ProntoSIL C4 | C4 | 60/120/200/300 | 3/5 | 7.5 - 2.5 | 1 - 8 | ProntoSIL C4 is a classical C4-type stationary phase; it is fully endcapped. Due to the bonding technology it shows an enhanced stability even at pH 1. The C4-packings show excellent properties for the separation of large biomolecules like proteins and peptides not only in the RP-mode but also in the HIC-mode. |
| ProntoSIL C1 | C1 | 120 | 3/5 | 3 | 1 - 8 | The C1 packing shows the lowest retention of the complete ProntoSIL line. The application area is mainly the separation of non-polar solutes. It can also be used for the separation of proteins in the HIC-mode. Due to the bonding technology the C1-bonding type is stable down to pH 1. |
| ProntoSIL C30 | C30 | 120/200/300 | 3/5/10 | 25 - 13 | 1 - 8 | ProntoSIL C30 is a stationary phase with a high carbon load. The high coverage of the support results in a very dense bonding and in an excellent shape selectivity and stability even at pH 1. Especially the wide pore supports are showing an enhanced shape selectivity. The application field of the C30 packings is the separation of isomers of carotenoids and other long alkyl chain solutes that can not be separated on classical C18 columns. |
| ProntoSIL Phenyl | Phenyl | 60/120 | 3/5 | 12 - 9.5 | 1 - 8 | ProntoSIL Phenyl is a RP packing that offers different selectivities in comparison to brush type stationary phases like C18 or C8; it is fully endcapped. Due to the bonding technology it shows excellent stability even at pH 1. |
| ProntoSIL Amino | NH2 | 120 | 3/5/10 | 4 | 3 - 8 | This phase can be used in three modes: NP-mode, RP-mode and IC-mode. In the NP-mode it is an alternative to the silica packings but offers different selectivities. In the RPmode the packing is mainly used for carbohydrate analysis. In the IC-mode the bonded phase can be used as a weak anion exchanger (WAX) for the analysis of anions and organic acids. The packing shows excellent properties in Supercritical Fluid Chromatography (SFC) where dense gases (i.e. CO2) are used as mobile phases. |
| ProntoSIL Amino E | NH2 | 120 | 5 | 5 | 3 - 8 | In comparison to ProntoSIL Amino this support is fully endcapped. Due to this, it is perfectly suitable for the SFC of basic compounds. |
| ProntoSIL Amino H | NH2 | 120 | 5 | 4.5 | 3 - 8 | This packing shows a different selectivity in Supercritical Fluid Chromatography (SFC). Due to a special bonding technology it shows a higher lifetime in carbohydrate analysis in the RP-mode. |
| ProntoSIL CN | CN | 120 | 3/5 | 6 - 5 | 3 - 8 | In RP-mode, the application area of ProntoSIL CN is the separation of strong basic solutes. In NPmode, the ProntoSIL CN-support offers a complementary selectivity to the other NP-phases: Silica, Amino and DIOL. Due to the quick equilibration time of the CN-bonded phase it is the best choice for gradient elution in NPmode. |
| ProntoSIL OH | DIOL | 60/120 | 3/5/10 | 5.5 - 4 | 3 - 8 | ProntoSIL OH is a DIOL bonded phase. The DIOL packing can be used in Normal Phase (NP) and Reversed Phase (RP) Chromatography. It is also suitable for Supercritical Fluid Chromatography (SFC). The silanophilic activity is lower than that of pure silica. This is particularly advantageous in Size Exclusion Chromatography (SEC). When the DIOL phase is employed in NP mode, the equilibration times are shorter than on a pure silica support, the selectivities, however, are similar. |
| ProntoSIL Enviro PHE | proprietary | 120 | 3 | 9.5 | 3 - 8 | ProntoSIL Enviro PHE is a special stationary phase for the determination of Phenols in environmental analysis. This stationary phase was designed to meet the requirements of EPA method 604/625. In this procedure 11 phenols have to be separated. The columns are pretested with a standard mixture described in EPA method 604/625. |
| ProntoSIL Enviro PTL | proprietary | 60 | 5 | 2.5 | 3 - 8 | ProntoSIL Enviro PTL is a special stationary phase for the group determination of non-aromatic, monoaromatic, diaromatic and polyaromatic hydrocarbons in petroleum products. This stationary phase was designed to meet the requirements of European Norm prEN 12916 (June 1997). In this norm a resolution between Cyclohexane (non-aromatic) and o-Xylene (monoaromatic) of minimum 7 is required. Therefore the columns packed with this stationary phases are only offered in the length of 250 mm. The columns are pretested with a standard mixture described in prEN 12916. |
| Enviro PES | proprietary | 100 | 5 | 15 | 3 - 8 | ProntoSIL Enviro PES is a special stationary phase for the determination of pesticides in environmental analysis. This stationary phase was designed to meet the requirements of German industry norm DIN 38407 F12. In this procedure 17 pesticides have to be separated. ProntoSIL Enviro PES columns are able to separate this complex mixture in less than 45 minutes. The columns are shipped pretested with the pesticide standard mixture. |
| PolyEncap A | C4 | 120/300 | 3/5/7/10 | 8 - 4 | 3 - 10 | PolyEncap stationary phases are a family of bonded phases where the silica is polymeric encapsulated. The encapsulation is done by acrylic acid ester polymers which are additionally covalent bonded to the silica surface. This type of stationary phase offers different selectivities compared to classical bonded brush type stationary phases. PolyEncap A has a comparable selectivity to a brush type C8 packing. |
| PolyEncap B | C18 | 120 | 3/5 | 14 | 3 - 10 | PolyEncap stationary phases are a family of bonded phases where the silica is polymeric encapsulated. The encapsulation is done by acrylic acid ester polymers which are additionally covalent bonded to the silica surface. This type of stationary phase offers different selectivities compared to classical bonded brush type stationary phases. PolyEncap B has a comparable selectivity to a brush type C18 packing. |
| Commercially equivalent phases | ||||||
| Hypersorb ODS | C18 | 120 | 3/5 | 9.5 | 3 - 8 | ProntoSIL Hypersorb ODS is the recommended alternative for Hypersil ODS. It is based on a type A silica and has the same bonding density like the original RP support. Due to a slightly lower acidy of the silica the silanophilic activity of the packing is a little bit lower compared to Hypersil ODS. The polarity of the surface is comparable. In the application of these stationary phases the same selectivity but better peak shape for the elution of basic compounds are observed. |
| Spheribond ODS 1 | C18 | 80 | 3/5/10 | 7 | 3 - 8 | ProntoSIL Spheribond ODS 1 is the recommended alternative for Waters Spherisorb ODS 1. It is based on a type A silica and has the same physical chemical properties and bonding density like the original RP support. The selectivity of the two packings is the same. |
| Spheribond ODS 2 | C18 | 80 | 3/5/10 | 12 | 3 - 8 | ProntoSIL Spheribond ODS 2 is the recommended alternative for Waters Spherisorb ODS 2. It is based on a type A silica and has the same physical chemical properties and bonding density like the original RP support. The selectivity of the two packings is the same. The typical application field of this classical packing with moderate silanophilic activity is pharmaceutical analytes. The Waters Spherisorb ODS 2 column is the most popular spherical silica support in pharmaceutical applications. |
| KromaPlus C18 | C18 | 110 | 3.5/5/10 | 20 | 1 - 10 | ProntoSIL KromaPlus - the Kromasil ODS alternative - is based on ProntoSIL Silica and is available for analytical and preparative HPLC. Synthesized with our special bonding technology, an ultra-pure phase with very high batch-to-batch reproducibility and high chemmical stability is obtained. The bonding type is monofunctional and the phase is end-capped. |
| HIPAK ODS AB | C18 | 130 | 3/5 | 11 | 3 - 8 | HIPAK is the typical second generation packing of Bischoff Chromatography. It is based on a type A silica but with additional base deactiviation. This packing is our recommended alternative to Hypersil BDS. |
| HIPAK C8 AB | C8 | 130 | 3/5 | 7 | 3 - 8 | HIPAK is the typical second generation packing of Bischoff Chromatography. It is based on a type A silica but with additional base deactiviation. This packing is our recommended alternative to Hypersil BDS. |
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