The most effective set of immobilized chiral columns, CHIRALPAK IA, IB, IC, and IG are used for primary screening. It is noteworthy that the four best columns are all meta-substituted.
The traditional CHIRALCEL and CHIRALPAK polysaccharide phases of Daicel consist of a derivatized cellulose or amylose physically coated onto a silica support. They are classified as Type II in the Wainer classification system and a wide range of column types are available. The ChromTech protein-based columns are suitable for all types of analytes - basic compounds can be separated on both CHIRAL-AGP and CHIRAL-CBH, acidic and neutral compounds can be separated on both CHIRAL-AGP and CHIRAL-HSA.

Primary Screening Columns
CHIRALPAK IA: Amylose tris (3,5-dimethylphenylcarbamate)
CHIRALPAK IB: Cellulose tris (3,5-dimethylphenylcarbamate)
CHIRALPAK IC: Cellulose tris (3,5-dichlorophenylcarbamate)
CHIRALPAK ID: Amylose tris (3-chlorophenylcarbamate)
CHIRALPAK IG: Amylose tris (3-chloro-5-methylphenylcarbamate)
CHIRALPAK IH: Amylose tris [(S)-alpha-methylbenzylcarbamate]
CHIRALPAK IJ: Cellulose tris (4-methylbenzoate)
CHIRALPAK IK: Cellulose tris(3-chloro-5-methylphenyl)carbamate
CHIRALPAK IM: Cellulose tris(3-chloro-4-methylphenyl)carbamate

Application of polysaccharide phases

most used

Instruction Manuals

ChromTech CSPs

CHIRAL-AGP is most likely the column with the broadest applicability of all chiral columns available today. The column is used in the reversed-phase and can be used for direct resolution of enantiomers, without derivatization. The CHIRAL-AGP column separates enantiomers of an extremely broad range of drug compounds: amines (primary, secondary, tertiary, and quaternary), acids (strong and weak) and nonprotolytes (amides, esters, alcohols, sulphoxides, etc.). Very simple mobile phase systems are used for the majority of the separations - buffered waterbased mobile phases with the addition of low concentrations of an organic modifier, such as 2-propanol or acetonitrile. Most of the separations (>95%) have been performed using the above described simple mobile phases. However, in some cases charged modifiers as DMOA (N,N-dimethyloctylamine), octanoic acid and quaternary ammonium compounds are used to induce enantioselectivity.

CHIRAL-CBH is preferentially used for the separation of enantiomers of basic drugs from many compound classes. This chiral column separates compounds containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.). The mobile phases are mixtures of phosphate or acetate buffers and organic solvents as 2-propanol or acetonitrile containing 50mM disodium EDTA. The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and organic modifier.

CHIRAL-HSA is especially suited for the resolution of weak and strong acids, zwitterionic and nonprotolytic compounds. However, basic compounds have also been resolved. The column is operated in the reversed phase mode. Phosphate buffers with addition of organic modifiers are used as mobile phases. Retention and enantioselectivity can be regulated by changing the mobile phase composition. The addition of octanoic acid is necessary when chromatographing for example arylpropionic acid derivates on CHIRAL-HSA.

Fast Screening on Chiral AGP, CBH and HSA

 Ampholyte is a substance that can act as either an acid, in the presence of a strong base, or a base, when in the presence of a strong acid.

Mobile Phases

Group 1    AGP – 10mM (NH₄ or Na)acetate buffer pH 4.5    CBH – 5% 2-propanol in 10mM Na-phosphate buffer pH 6.0

Groups 2, 3 and 5   ALL – 5% 2-propanol in 10mM Na-phosphate buffer pH 7.0

Groups 4 and 6    ALL – 10mM Na-phosphate buffer pH 7.0