ACE
A new generation of ultra pure base deactivated columns
from Advanced Chromatography Technologies (ACT)
- Ultra high purity base deactivated silica
- 2um, 3um, 5um, 10um and 15um particle sizes
- Column diameters 0.3 .... 30mm
- Fully validated columns
- Exceptional reproducibility
- Excellent efficiency (up to 200,000
plates/metre)
ACE phases
| Phase | Functional group | Endcapped | Particle, um | Pore, A | Carbon, % |
| C4 | C4 | Yes | 2, 3, 5, 10 | 100 | 5.5 |
| C4-300 | C4 | Yes | 3, 5, 10 | 300 | 2.6 |
| C8 | C8 | Yes | 2, 3, 5, 10 | 100 | 9.0 |
| C8-300 | C8 | Yes | 3, 5, 10 | 300 | 5.0 |
| C18 | C18 | Yes | 1.7, 2, 3, 5, 10 | 100 | 15.5 |
| C18-HL (high load) | C18 | Yes | 3, 5, 10, 15 | 90 | 20.0 |
| AQ (aqueous) | C18 | Yes | 2, 3, 5, 10 | 100 | 14.0 |
| SuperC18 | C18 | Encapsulated | 1.7, 2, 3, 5, 10 | 90 | 14.8 |
| C18-Amide | C18-Amide | Yes | 1.7, 2, 3, 5, 10 | 100 | 16.4 |
| C18-AR | C18-Phenyl | Yes | 1.7, 2, 3, 5, 10 | 100 | 15.5 |
| C18-PFP (PentaFluoroPhenyl) | C18-PF-Phenyl | Yes | 1.7, 2, 3, 5, 10 | 100 | 14.3 |
| C18-300 | C18 | Yes | 3, 5, 10 | 300 | 9.0 |
| Phenyl | Phenyl | Yes | 2, 3, 5, 10 | 100 | 9.5 |
| Phenyl-300 | Phenyl | Yes | 3, 5, 10 | 300 | 5.3 |
| CN | CN | Yes | 2, 3, 5, 10 | 100 | 5.5 |
| CN-ES | CN-alkyl | Yes | 1.7, 2, 3, 5, 10 | 100 | 12.6 |
| NH2 | NH2 | No | 1.7, 3, 5 | 100 | 4.0 |
| CN-300 | CN | Yes | 3, 5, 10 | 300 | 2.6 |
| Silica | Si | --- | 2, 3, 5, 10 | 100 | --- |
| HILIC-A | Si | --- | 1.7, 3, 5 | 100 | --- |
| HILIC-B | Aminopropyl | --- | 1.7, 3, 5 | 100 | 4.0 |
| HILIC-N | Polyhydroxy | --- | 1.7, 3, 5 | 100 | 7.0 |
Basic validation parameters (C18 column, 250 x 4.6mm x 5um)
| Specification | Result | ||
| Peak Asymmetry - Amitriptyline | % | >90 | 97 |
| As0.1 | <1.30 | 1.02 | |
| USP Tailing Factor | <1.20 | 1.05 | |
| Selectivity | k’(Phenanthrene) | 4.08 - 4.42 | 4.35 |
| k’(Desipramine) / k’(Phenanthrene) | 0.120 - 0.130 | 0.125 | |
| k’(Doxepin) / k’(Phenanthrene) | 0.340 - 0.368 | 0.352 | |
| k’(Imipramine) / k’(Phenanthrene) | 0.496 - 0.538 | 0.517 | |
| k’(Amitriptyline) / k’(Phenanthrene) | 0.654 - 0.708 | 0.678 |
Mobile Phase: 80:20 MeOH / 25mM KH2PO4
(pH 6.0)
Flow Rate: 1.0ml/min,
Temperature: 22°C, Wavelength: 215nm
Batch Validation Report, Test Chromatogram
see
Downloads ... Misc.
Acidic-basic resistance
pH 1.8 Robustness
The high stability of ACE C18, C8, C4, CN and Phenyl phases was
demonstrated: after three months usage at pH 1.8, no retention loss was
observed with any ACE phase. Even cyano bonded columns (regarded to be most
vulnerable to hydrolysis) showed excellent resistance.
pH 11.0 Robustness
Again, the ultra high purity ACE silica coupled with unique dense bonding
technology effectively prevents silica dissolution. ACE columns withstand
highly alkaline conditions without loss of column efficiency after 3 months
usage at pH 11.0.
ACE capillary and nano columns
- High sensitivity
- Low sample mass and volume application
-
Compatible with LC-MS and LC-MS/MS
- Very low solvent consumption
- Suitable for proteomics
- Compatible with LC-MS and LC-MS/MS
ACE UltraCore phases
| Phase | Functional group | Endcapped | Particle, um | Pore, A | Carbon, % |
| C18 | C18 | Yes | 3.5 | 95 | 8.2 |
| Phenyl-Hexyl | Phenyl-Hexyl | Yes | 3.5 | 95 | 6.2 |
| Biphenyl | Biphenyl | Yes | 3.5 | 95 | 6.5 |
| C18-Amide | embedded Amide | Yes | 3.5 | 95 | 5.5 |
| SuperC18 | C18 | Encapsulated | 2.5 / 5.0 | 95 | 7.0 / 5.4 |
| SuperPhenylHexyl | Phenyl-Hexyl | Encapsulated | 2.5 / 5.0 | 95 | 4.6 / 3.6 |
| BIO C18 | C18 | Yes | 3.5 | 300 | 1.0 |
| BIO C18 | C18 | Yes | 2.5 | 500 | 1.4 |
| BIO C4 | C4 | Yes | 3.5 | 300 | 0.4 |
| BIO C4 | C4 | Yes | 2.5 | 500 | 0.6 |
| BIO Phenyl2 | Biphenyl | Yes | 3.5 | 300 | 0.7 |
| BIO Phenyl2 | Biphenyl | Yes | 2.5 | 500 | 1.0 |
News
ACE HILIC UHPLC and HPLC Columns - Three HILIC column chemistries available; Method Development Kits for the same price as a single column
ACE HILIC Brochure
ACE HILIC Guide
Step-by-Step Protocol for HILIC Method
Development
ACE Step-by-Step HILIC Method
Development Protocol (Flow Chart)
The Importance of Sample
Diluent in HILIC
Column Equilibration
in HILIC Mode
Selectivity
Differences on the ACE HILIC Range
Comparing
Selectivity in HILIC and RPLC Modes
A Simple, Generally Applicable
HILIC Method Development Platform Based upon Selectivity
A Practical, Selectivity Based
HILIC Method Development Protocol
Separation of Methylmalonic Acid and Succinic Acid using HILIC Mode
Separation of
beta-Blockers using HILIC Mode
Separation of Caffeine and
Related Compounds using HILIC Mode
Separation
of Adenine and Nucleosides using HILIC-MS Mode
Separation of
Nitrogenous Bases and Nucleosides using HILIC-MS Mode
Separation
of Creatine and Metabolite Creatinine using HILIC Mode
Catalogs
ACE HPLC and UHPLC Columns Catalog
2020
ACE HPLC and UHPLC Applications Guide
ACE UHPLC columns 1.7um
ACE HPLC Columns Catalog 2013
ACE HPLC Columns Catalog 2011/12
ACE HPLC Columns Catalog 2009/10
Brochures
ACE Method Development Kits
ACE SuperC18 Brochure
ACE C18-Amide Brochure
ACE CN-ES Brochure
ACE Excel flyer
ACE Excel brochure
How to install ACE Excel UHPLC
columns?
ACE UHPLC reusable column connector
ACE Generix Brochure
The Benefits of Ultra Inert Stationary Phases
for the Reversed Phase HPLC of Biomolecules - Ranking of 300A Columns
ACE Capillary and Nano Columns, LC-MS and Rapid
Analysis columns
ACE C18-PFP Brochure
ACE C18-AR Brochure
Improved HPLC Separation of Steroids
ACE Amino columns
ACE Excel Glycan columns
ACE Excel Oligo columns
FOCUS: Chromatography Magazine
Issue 2024.01
Issue 2023.02
Issue 2023.01
UltraCore Columns
ACE UltraCore Brochure
ACE UltraCore
SuperC18 MS Brochure
Buffer
Recommendations for ACE UltraCore Columns
ACE Applications by Market Sector
Beauty applications
Biomolecules
Clinical applications
Environmental applications
Food and beverage
applications
Forensic applications
Mass spectrometry applications
Petrochemical applications
Pharmaceutical
applications
USP monographs
Posters, Presentations
Exploring
and Leveraging Multi-Mode Interactions to Maximise Chromatographic
Selectivity with Uniquely Designed HPLC / UHPLC Stationary Phases
Exploring HPLC /
UHPLC Chromatographic Selectivity with Low And High pH Eluents on a New
Extended pH Range Stable Silica-Based Stationary Phase
Exploring Low and High pH Eluents Using a New Silica-Based Extended pH Range
C18 Column for Analysis of Amphetamines and Opiates from Urine by LC-MS
Exploring the Power
of Chromatographic Selectivity for Polar and Non-Polar Analytes with a
Unique UHPLC / HPLC Polar Embedded Phase
Application of a
Unique HPLC Phase when Exploring HPLC Method Development Challenges for a
Pharmaceutical Combination Therapy Containing Five Active Ingredients
Low Level Determination of Synthetic Cannabinoids (SPICE) and Metabolites
from Oral Fluid Using a Novel C18-Based Stationary Phase by UHPLC-MS/MS
Exploring the
Selectivity and Performance of a New Extra Selectivity / Extended Stability
Cyano Phase in UHPLC / HPLC Method Development
A New,
Extended pH Range Stable Solid Core UHPLC / HPLC Column Family with SuperC18
and SuperPhenylHexyl Bonded Phases
Using
Selectivity Data to Demonstrate a UHPLC / HPLC Method Development Platform
Based upon Solid Core SuperC18 and SuperPhenylHexyl Phases
ACE
C18-AR and C18-PFP: rational stationary phase design
Introducing reproducible selectivity changes to HPLC
Determination of
Pesticides in Foodstuffs by LC-MS/MS (ACE UltraCore SuperC18)
Translating US Pharmacopoeia Methods to
Sub-2 Micron and Solid Core Using the New USP <621> General Chapter
Guidelines
Managing Method Transfer in the
Pharmaceutical Laboratory
Method for the
Determination of NDMA in Ranitidine Drug Substance and Drug Product (ACE
C18-AR)
Guides
Buffer Selection Guide
Avantor ACE Peptide and
Proteine
Guide
Proteins and Peptides Purifications
Guide
HPLC Troubleshooting Guide
C18 Comparison Guide
ACE Applications Guide
ACE Food and Beverage Applications Guide
ACE Clinical,
Forensic and Bioanalysis Applications Guide
ACE Cannabis Applications Guide
ACE Knowledge Notes
0001 - How to Determine System
Dwell Volume: Theory and Practice
0002 - Retention Factor, k
0003 - How to Calculate
Resolution
0004 - Column Care, Cleaning and
Storage
0005 - The Fundamental
Resolution Equation and Impact of k, N and α
0006 - UHPLC Column
Connections
0007 - Gradient
Chromatography and k*
0008 - The Role of Methanol
and Acetonitrile as Organic Modifiers in Reversed-phase Liquid
Chromatography
0009 - The Effect of Sample
Diluent on Peak Shape
0010 - Chromatographic Band
Broadening and the van Deemter Equation
0011 - Practical Ultra High
Performance Liquid Chromatography (UHPLC)
0012 -
Understanding the Relationship between Particle Size, Performance and
Pressure
0013 - The Power of Stationary
Phase Selectivity
0014 - Analyte Properties:
pKα, logP and logD
0015 - How to Determine
Column Volume and Porosity
0016 - USP Column Codes
0017 - How to Determine
Extra Column Dispersion and Extra Column Volume
0018 - Step-by-Step Protocol for
Streamlined Reversed-phase Method Development using Avantor ACE MDKs
0019 - Avantor ACE UltraCore and Solid Core Technology
0020 - Increasing HPLC /
UHPLC Sample Throughput: Doing More in Less Time
0021 - A Simple Step-by-Step
Protocol for HILIC Method Development
0022 - Creating the Correct
Environment for Analysing Reducing Sugars with the Avantor ACE NH2
0023 - Gradient
Method Translation Using the Avantor ACE LC Translator
0024 - The Importance of
Sample Diluent in HILIC
0025 - Column
Equilibration in HILIC Mode
0026 - Using Narrow Bore Columns
to Enhance Sensitivity for LC-MS Analyses
0028 - Transferring LC
Methods Between Different Instruments Using the Avantor ACE LC Translator
Tool
0029 - Allowable Changes
to USP and Ph.Eur. Monograph LC Methods
0030 - Avantor ACE UHPLC Guards
0031 - The Use of Mobile Phase pH as a
Method Development Tool
0033 - The Importance of Column
Temperature in Liquid Chromatography
0034 - Approaches for Reducing
the Environmental Impact and Increasing Throughput of LC Separations
0035 - Reducing the LC Column's
Internal Diameter to Drive Sustainable Chromatography
0036 - December 2022 Revision to USP
<621> Allowable Changes to Monograph LC Methods
0038 - The Scale-up Process: from
Analytical to Preparative Scale LC Separations
ACE LC Translator Tools
ACE LC Translator
ACE LC Translator Help
ACE Pharmacopoeia
LC Translator
ACE
Pharmacopoeia LC Translator Help
Misc.
ACE Stationary Phases
ACE Stationary Phases and Part Numbers
ACE Newsletter 1.
ACE Newsletter 2.
Reproducibility and Validation of ACE HPLC /
UHPLC Columns
ACE Batch Validation Report
ACE Column Test Report
Solvent exchange between NP - RP
phases
HPLC Column Regeneration
ACE Precolumn Filters
ACE Method Transfer Calculator (xls file)
